ICSI Treatment Mumbai
ICSI Treatment Mumbai,Intracytoplasmic sperm injection is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg. This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. With this method, the acrosome reaction is skipped. ICSI is used in 95% of oocytes in the world.
There are several differences between classic IVF and ICSI. However, the steps to be followed before and after insemination are the same. In terms of insemination, ICSI needs one only sperm cell per oocyte, meanwhile, IVF needs between 50 and 100 thousandths are due to the fact that in IVF acrosome reaction has to take place and thousands of sperm cells have to be involved. Once fertilized, the egg is transformed into a proembryo and it has to be transferred to the uterus to continue its development.
This procedure is most commonly used to overcome male infertility problems, although it may also be used where eggs cannot easily be penetrated by sperm, and occasionally in addition to sperm donation.
It can be used in teratozoospermia because once the egg is fertilized, abnormal sperm morphology does not appear to influence blastocyst development or blastocyst morphology.[6] Even with severe teratozoospermia, microscopy can still detect the few sperm cells that have “normal” morphology, allowing for optimal success rate.
It can also be used in Azoospermia, “Various spermatozoa” (from Fertility Preservation after cancer, or because of a fertilization failure after IVF.
ICSI Treatment Mumbai is generally performed following a transvaginal oocyte retrieval procedure to extract one to several oocytes from a woman.
In ICSI Treatment Mumbai IVF, the male partner or a donor provides a sperm sample on the same day when the eggs are collected.[16] The sample is checked in the lab, and if no sperm is present, doctors will extract sperm from the epididymis or testicle. The extraction of sperm from epididymis is also known as percutaneous epididymal sperm aspiration (PESA) and extraction of sperm from testicle is also known as testicular sperm aspiration (TESA).
The procedure is done under a microscope using multiple micromanipulation devices (micromanipulator, microinjectors, and micropipettes). A holding pipette stabilizes the mature oocyte with gentle suction applied by a microinjector. From the opposite side a thin, hollow glass micropipette is used to collect a single sperm, having immobilized it by cutting its tail with the point of the micropipette. The oocyte is pierced through the oolemma and the sperm is directed into the inner part of the oocyte (cytoplasm). The sperm is then released into the oocyte. The pictured oocyte has an extruded polar body at about 12 o’clock indicating its maturity. The polar body is positioned at the 12 or 6 o’clock position, to ensure that the inserted micropipette does not disrupt the spindle inside the egg. After the procedure, the oocyte will be placed into cell culture and checked on the following day for signs of fertilization.
In contrast, in natural fertilization sperm compete and when the first sperm penetrates the oolemma, the oolemma hardens to block the entry of any other sperm. Concern has been raised that in ICSI Treatment Mumbai this sperm selection process is bypassed and the sperm is selected by the embryologist without any specific testing. However, in mid-2006 the FDA cleared a device that allows embryologists to select mature sperm for ICSI Treatment Mumbai based on sperm binding to hyaluronan, the main constituent of the gel layer (cumulus oophorus) surrounding the oocyte. The device provides microscopic droplets of hyaluronan hydrogel attached to the culture dish. The embryologist places the prepared sperm on the microdot, selects and captures sperm that binds to the dot. Basic research on the maturation of sperm shows that hyaluronan-binding sperm is more mature and show fewer DNA strand breaks and significantly lower levels of aneuploidy than the sperm population from which they were selected. A brand name for one such sperm selection device is PICSI.[17] A recent clinical trial showed a sharp reduction in miscarriage with embryos derived from PICSI sperm selection.[18]
